A: Representative photomicrograph showing FG-positive cells (dark brown color cells) and Ucn 3 mRNA-positive signals (black dot clusters) in the PVHap. B: High power magnification of boxed area in A showing colocalization of FG immunoreactivity and Ucn 3 mRNA signals. C,D: Photomicrographs of B at different focal planes showing FG-positive cells (C) and Ucn 3 mRNA signals (silver grain clusters, D). Representative double-labeled cells are indicated by red arrowheads. Representative single-labeled FG-positive cells in C are indicated by blue arrowheads and single-labeled Ucn 3 mRNA-positive cells in D are indicated by yellow arrowheads. 3V: Third ventricle, f: Fornix,, PVHap: anterior parvicellular part of the paraventricular nucleus of hypothalamus. Scale bar: 50 μm.