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Representative stacked confocal images showing Ucn 3 (A) and Enk (B) immunostaining in the perifornical area indicated in D (red box). C: Merged images of Ucn 3 (A) and Enk (B) showing high degree of colocalization of the two materials in neurons and fibers in this brain area. 3V: Third ventricle, AH: Anterior hypothalamus, f: Fornix, ic: Internal capsule, mt: Mammillothalamic tract, PVH: Paraventricular nucleus of hypothalamus. opt: Optic tract. Scale bar: 25 μm.
