Table 1.
Abbreviated list of examples of ZFN, TALEN and CRISPR/Cas-mediated genome editing in human cells and model organisms.
| Type of modification |
Organism | Gene(s) | Nuclease(s) | Ref(s) |
|---|---|---|---|---|
| Gene disruption | Human | CCR5 | ZFN | [66, 92, 93] |
| TALEN | [26, 53] | |||
| CRISPR/Cas | [102] | |||
| Human | TCR (T-cell receptor) | ZFN | [95, 96] | |
| Zebrafish |
gol (Golden), ntl (No tail), kra |
ZFN | [67, 69] | |
| Pig |
GGTA1 (α1, 3- galactosyltransferase) |
ZFN | [78] | |
| LDLR (LDL receptor) | TALEN | [77] | ||
| Bovine | ACAN12, p65 | TALEN | [77] | |
| Human | EMX1, PVALB | CRISPR/Cas | [103] | |
| Rat | IgM, Rab38 | ZFN | [71] | |
| Arabidopsis | ADH1, TT4 | ZFN | [82] | |
| C. elegans | ben-1, rex-1, sdc-2 | ZFN/TALEN | [79] | |
| Hamster | DHFR | ZFN | [38] | |
| Drosophila | yellow | ZFN | [73] | |
| Rice | OsSWEET14 | TALEN | [85] | |
| Gene addition | Human | OCT4, PITX3 | ZFN/TALEN | [46, 47] |
| Human | CCR5 | ZFN | [98] | |
| Human |
F9 (Coagulation Factor IX) |
ZFN | [87] | |
| Mouse | Rosa26 | ZFN | [58] | |
| Human | AAVS1 | ZFN | [46, 97, 98] | |
| TALEN | [47] | |||
| CRISPR/Cas | [104] | |||
| Human | VEGF-A | ZFN | [18] | |
| Zebrafish |
th (tyrosine hydroxylase), fam46c smad5 |
TALEN | [81] | |
| Maize | IPK1 | ZFN | [83] | |
| Gene correction | Human | IL2RG | ZFN | [45, 86] |
| A1AT (α1-antitrypsin) | ZFN | [90] | ||
| HBB (β-globin) | ZFN | [88, 89] | ||
| SNCA (α-synuclein) | ZFN | [91] | ||
| Tobacco |
SuRA, SurRB (acetolactate synthase) |
ZFN | [84] | |
| Drosophila | yellow | ZFN | [72] |