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Immunohistochemical and pH differences between treated and untreated animals. A, immediately before sacrifice prostate tumor showed significant increase in prostate pH in mice that started bicarbonate (Bicarb) at age 4 vs 10 weeks and vs mice on tap water during experiment. B, immunohistochemistry staining for CAIX expression in normal prostate tissue of control and treated mice reveal stronger staining in untreated mice.
