FIG. 1.

Construction of the HCMV IE2 86 deletion mutant BACs. (A) Schematic of exon 5 of the HCMV MIE region. For construction of the four mutants, IE2 86Δ356-359, IE2 86R356/7/9A, IE2 86Δ427-435, and IE2 86Δ505-511, regions of the exon were deleted or amino acids were substituted as shown. Zn, zinc finger domain; HLH, helix-loop-helix motif. (B) EcoRI digests of the HCMV BAC clones. Three micrograms of wild-type (pHB5), mutant, or rescued mutant BAC DNA was cut with EcoRI, and the resulting digests were separated on a 0.6% agarose gel in 1× Tris-borate-EDTA. MW, molecular weight markers.