FIG. 2.

Increased IE1 and IE2 transcription at IE times in cells electroporated with the IE2 86 mutant BACs. G₀-synchronized HFFs were electroporated with pHB5, IE2 86Δ356-359, IE2 86R356/7/9A, IE2 86Δ427-435, or IE2 86Δ505-511 BAC and pcDNApp71tag and were harvested at the indicated time points postelectroporation. Total cellular RNA was analyzed by quantitative real-time RT-PCR as described in Materials and Methods to measure the presence of IE1 72 (A) or IE2 86 (B) transcripts. Values displayed in the graphs are the averages of two to four independent experiments, with each conducted using duplicate reactions. Error bars indicate 1 standard deviation from the means of the combined experiments. To ensure that an equal amount of RNA was included in each reaction, we analyzed samples with G6PD-specific primers and probe (C).