FIG. 4.

IE1 72 and IE2 86 expression in wild-type- and IE2 86 mutant BAC-electroporated cells at 9 days postelectroporation. Cells were seeded onto sterile coverslips in 12-well dishes after electroporation with pcDNApp71tag and pHB5, IE2 86Δ356-359(A), IE2 86Δ505-511 (A), or IE2 86Δ427-435 (B), and 9 days later they were fixed with 2% paraformaldehyde in PBS. Coverslips were processed as described above (in the legend for Fig. 3 and Materials and Methods). Magnification, ×400.