FIG. 7.

UL89 and R160461 transcription is increased at IE times in mutant BAC-electroporated cells. Total cellular RNAs were isolated from electroporated cells as described in Materials and Methods and the legend for Fig. 2 and were analyzed by quantitative real-time RT-PCR with UL89 (A)- or R160461 (B)-specific primers and TaqMan dual-labeled probes. Values displayed in the graphs are the averages of two to four independent experiments, with each conducted using duplicate reactions. Error bars indicate 1 standard deviation from the means of the combined experiments.