FIG. 8.

pp28 expression in mutant BAC-electroporated cells. Cells were seeded onto sterile coverslips in 12-well dishes after electroporation with pcDNApp71tag and pHB5 or IE2 86Δ356-359, and 9 days later they were fixed with 2% paraformaldehyde in PBS. Coverslips were stained with Hoechst dye to visualize nuclei and with anti-pp28 monoclonal antibody 41-18 followed by FITC-conjugated anti-mouse secondary antibody. The blank field indicating the lack of pp28 staining in the IE2 86Δ356-359-electroporated cells is included for comparison and is representative of pp28 staining in cells electroporated with the IE2 86Δ427-435 or IE2 86Δ505-511 BAC. Magnification, ×400.