Figure 3.

Multiple Gene Silencing in Arabidopsis by a Single Optimal AmiRNA.

(A) Schematic diagram of amiR-RACK1 (red) for silencing three target genes, RACK1a, 1b, and 1c, of the RACK1 family.

(B) Immunoblot of RACK1-HA proteins to define optimal amiR-RACK1. The numerical order of each amiRNA was based on the high-to-low WMD ranking, and the optimal amiR-RACK1-4 is highlighted in red. Three independent repeats with GFP-HA as an untargeted internal control produced similar results. Ctrl, control.

(C) Transgenic Arabidopsis plants overexpressing amiR-RACK1-4 resemble the rack1a,1b,1c triple null mutant. WT, the wild type.