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. 2013 May 3;25(5):1507–1522. doi: 10.1105/tpc.113.112235

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Figure 7. — Analysis of the Distribution of Optimal AmiRNAs.

(A) Scatterplot of the amiRNA efficacy and the distance of amiRNA target site to the translation initiation site within target mRNAs. Optimal amiRNAs reach ∼90% protein silencing efficacy (Table 1). The number of optimal amiRNAs versus the total number of candidates with the target site located <200 or >200 nucleotides (nt) from the start codon in the mRNA is shown at the top.

(B) Distribution of optimal amiRNAs among candidates with different mismatch numbers. The number of optimal amiRNAs versus the total number of candidates is shown on top of the column for the indicated mismatch number.

(C) Scatterplot of the ratio of hybridization energy (dG_amiR-target/dG_{perfect-match}) of an amiRNA and its efficacy. The number of optimal amiRNAs versus the total number of candidates with the ratio of hybridization energy within 70 to 80%, 80 to 90%, or 90 to 100% is shown at the top.