Table 1. Model Constraints and Model Predicted Exchange Fluxes and Other Rates for Various Organs of Developing Oilseed Rape Embryos in Planta.
| A | B | C | ||
|---|---|---|---|---|
| Sub-Model (In Planta, 32 DAF) | ||||
| Flux value constraints | Radicle/Hypocotyl | Inner Cotyledon | Outer Cotyledon | Units |
| Growth rate (Biomass_exch) a | 0.0005 | 0.00136 | 0.00226 | mg DW h−1 |
| Photon uptake flux (Ph_tm_exch) | 0.014222 | 0.0022556 | 0.07781 | μmol h−1 |
| ATPdrain (ATP_c:H2O_c::ADP_c:Pi_c) | 0.014905 | 0.055864 | 0.098175 | μmol h−1 |
| Nitrate uptake (NO3_ap_exch) | 0 | 0 | 0 | μmol h−1 |
| Ammonia uptake (NH4_ap_exch) | 0 | 0 | 0 | μmol h−1 |
| Biomass composition b | ||||
| Total lipids | 34.8 | 40.8 | 42.8 | % DW |
| Total proteins | 24.2 | 21.4 | 21.8 | % DW |
| DNA | 0.10 | 0.10 | 0.10 | % DW |
| RNA | 0.10 | 0.10 | 0.10 | % DW |
| Starch | 3.35 | 3.07 | 5.06 | % DW |
| Free metabolites | 10.00 | 10.00 | 10.00 | % DW |
| Cell wall (glucose polymer) | 27.42 | 24.48 | 20.15 | % DW |
| Fatty acid composition, mid stage b | ||||
| C16:0 (C16H31O2) | 11.49 | 7.13 | 6.65 | g/ 100 g fatty acids |
| C18:0 (C18H35O2) | 2.33 | 2.95 | 2.47 | g/ 100 g fatty acids |
| C18:1 (C18H33O2) | 41.73 | 51.35 | 47.13 | g/ 100 g fatty acids |
| C18:2 (C18H31O2) | 31.36 | 24.71 | 27.88 | g/ 100 g fatty acids |
| C18:3 (C18H29O2) | 12.12 | 12.57 | 14.49 | g/ 100 g fatty acids |
| C20:0(C20H39O2) | 0.55 | 0.62 | 0.62 | g/ 100 g fatty acids |
| C20:1(C20H37O2) | 0.42 | 0.67 | 0.76 | g/ 100 g fatty acids |
| Model predicted exchange fluxes and flux ratios c | ||||
| Gln uptake | −0.00082 | −0.00200 | −0.00337 | μmol h-1 |
| Suc uptake | −0.00230 | −0.00749 | −0.01163 | μmol h-1 |
| CO2 exchange | 0.00779 | 0.03281 | 0.04365 | μmol h-1 |
| O2 exchange | −0.00297 | −0.01776 | −0.01759 | μmol h-1 |
| Relative Rubisco flux d | 0.000264 | 0.000203 | 0.000218 | NA |
| CCE e | 75.43% | 67.15% | 72.09% | NA |
| CCE (no light) | 69.70% | 66.87% | 66.07% | NA |
| Relative contribution of photosynthetic electron transport to demands of plastidic syntheses via NADPH, ferredoxin | 57.8% | 3.0% | 59.0% | NA |
| Relative contribution of light driven ATP production to plastidic ATP consumption | 78.6% | 4.1% | 80.6% | NA |
a
Reaction names, Supplemental Data Set 3.
b
Based on biomass composition, model specific equations for the reactions “TAGsynth_c”, “Lipase_c” and “ Biomasssynth_nc, ap, c, p” are specified in Suppl. Table 1.
c
Negative flux values denote influx.
d
as defined in Schwender et al. (2004)
e
carbon conversion efficiency
DAP, days after pollination; DW, dry weight; NA, not applicable.