Figure 1.

Identification of Null Mutant Lines for ABC1K1 (abc1k1-1 and abc1k1-2) and ABC1K3 (abc1k3).

(A) The structure of the ABC1K1 and ABC1K3 loci and the position of the T-DNA insertions. Exons are indicated by black boxes, introns are indicated by lines, and the 5′ and 3′ untranslated regions are not included. The diagonal hash marks indicate the location of the ABC1K domain (Lundquist et al., 2012b).

(B) RT-PCR amplification (35 cycles) of ABC1K1, ABC1K3, or ACTIN2 cDNA show that k1 and k3 mutants do not accumulate transcripts containing the C-terminal portions for ABC1K1 and ABC1K3, respectively. For information about primer location and additional RT-PCR analysis, see Supplemental Figure 1 online. wt, the wild type.

(C) Immunoblot with anti-ABC1K1 and ABC1K3 antisera of unstressed mature leaf tissue protein extracts. Thirty micrograms of protein was loaded per lane. The asterisk identifies a nonspecific product or a truncated ABC1K1 protein product. A Ponceau stain showing the region around RBCL is included as a loading control under each immunoblot.