Figure 10.

Metabolite Profiling of Thylakoids and PGs.

(A) The abundance of selected prenyl-lipids normalized to the total pool of prenyl lipids were plotted from presonicated thylakoid preparations, containing thylakoid and bound PGs (Thy+PG) as well as subsequent fractions of thylakoid and isolated PGs. wt, the wild type.

(B) HPLC chromatographic traces of wild-type and k1 k3 PGs. Separation of prenyl-lipids was done on a reverse-phase C30 column using a methyl t-butyl ether mobile phase. k1 k3 is offset from the wild type along the y axis. Identification of metabolite peaks was based on retention time and absorption spectrum, established by Fraser et al. (2000) with the exception of PC-8, PQ-9, and Quinone 1, identified by HPLC-MS analysis (see Supplemental Figure 12 online). Abundant peaks are indicated by number as follows: (1) quinone 1 (molecular mass = 746.6 D), (2) α-tocopherol, (3) phylloquinone, (4) plastochromanol-8 (reduced), (5) 15-cis-phytoene, (6) PQ-9 (oxidized), (7) chlorophyll b, (8) zeaxanthin, (9) chlorophyll a, (10) unidentified carotenoid, (11) β-carotene, and (12) 9-cis-β-carotene. mAU, milli Absorbance Units.