Figure 2.
abc1k1-1 and abc1k3 Demonstrate a Conditional and Synergistic Light Stress Phenotype.
(A) Mature, 3-week-old plants grown under a 120 µmol photons m−2 s−1 light regime were transferred to either 520 or 1000 µmol photons m−2 s−1 and photographed at the described time points. wt, the wild type. Bar = 2 cm.
(B) The abaxial face of leaves of the wild type and k1 k3 treated with (5×) light stress treatment for 7 d demonstrate the lack of anthocyanin accumulation outside of the vasculature in k1 k3. Bar = 2 cm.
(C) Immunoblot with anti-ABC1K1 and anti-ABC1K3 antisera of mature leaf tissue protein extracts harvested after 0, 1, or 5 d of light stress. Twenty micrograms of protein from each sample was separated by SDS-PAGE and immunoblotted with the indicated antibody. A Ponceau stain showing the region around the RBCL is included as a loading control.