Table 1. Quantitative Metabolite Profiles of Thylakoid and PG Prenyl Lipids in ng/μg Prenyl-Lipids.
| The Wild Type |
k1 k3 |
|||||
|---|---|---|---|---|---|---|
| Prenyl-Lipid Metabolite | Thy and PGa | Thya | PGa | Thy and PGa | Thya | PGa |
| Plastoquinone-9b | 104.1 ± 24.4 | 104.1 ± 16.8 | 193.8 ± 49.7 | 64.5 ± 28.6 | 46.7 ± 27.0 | 359.4 ± 53.7 |
| Phylloquinone | 11.1 ± 4.2 | 12.0 ± 6.6 | 4.9 ± 1.9 | 23.0 ± 2.5 | 24.1 ± 3.2 | 24.8 ± 7.9 |
| Quinone 1c,d | n.d. | n.d. | n.d. | n.d. | n.d. | 67.1 ± 70.5 |
| PC-8b | n.d. | n.d. | 289.4 ± 48.1 | n.d. | n.d. | 160.4 ± 76.6 |
| α-Tocopherol | 162.6 ± 42.6 | 82.6 ± 24.4 | 421.6 ± 75.4 | 131.4 ± 15.0 | 119.2 ± 59.9 | 324.3 ± 117.5 |
| γ-Tocopherol | n.d. | n.d. | n.d. | 10.4 ± 7.1 | 19.7 ± 19.1 | n.d. |
| Phytoenee | 0.4 ± 0.1 | 0.2 ± 0.3 | 0.9 ± 0.1 | n.d. | n.d. | 0.2 ± 0.1 |
| Lutein | 138.2 ± 7.0 | 186.4 ± 29.0 | 0.3 ± 0.2 | 132.2 ± 15.8 | 135.4 ± 19.4 | 4.4 ± 01.5 |
| Violaxanthin | 28.6 ± 4.2 | 38.8 ± 5.5 | 1.1 ± 0.7 | 15.1 ± 2.4 | 16.4 ± 4.3 | 3.4 ± 2.4 |
| Antheraxanthin | 16.3 ± 1.9 | 21.8 ± 2.6 | n.d. | 15.5 ± 3.1 | 17.6 ± 2.5 | n.d. |
| Zeaxanthin | 80.6 ± 28.4 | 111.1 ± 37.9 | 3.7 ± 0.8 | 74.3 ± 28.1 | 94.4 ± 12.7 | 14.2 ± 5.3 |
| Neoxanthin | 0.4 ± 0.1 | 0.3 ± 0.3 | n.d. | 0.1 ± 0.3 | 0.4 ± 0.1 | n.d. |
| α-Carotene | 4.0 ± 1.4 | 5.1 ± 1.7 | n.d. | 2.3 ± 0.3 | 2.4 ± 0.4 | n.d. |
| β-Carotene | 20.1 ± 7.0 | 24.4 ± 14.6 | 1.2 ± 0.6 | 16.0 ± 6.3 | 20.8 ± 8.5 | 10.5 ± 6.7 |
| Other prenyl-lipidsf | 421.8 ± 98.9 | 367.4 ± 23.9 | 83.2 ± 22.0 | 324.6 ± 15.3 | 330.0 ± 7.2 | 30.6 ± 16.1 |
n.d., metabolite not detected.
a
Lipids were extracted from thylakoid samples before and after sonication, which included both thylakoids and attached PGs or thylakoids from which most PGs were removed. PG are isolated PGs.
b
Oxidized and reduced forms.
c
Unknown compounds were tentatively assigned to a compound class based upon their absorption spectrum.
d
The molecular mass of quinone 1 was determined by MS to be 746.6 D.
e
Cis- and trans-forms.
f
Other prenyl-lipids include chlorophyll a and b, two unknown tocopherol compounds, eight unknown carotenoid compounds, and seven unknown quinone compounds, which were assigned to a compound class based on their absorption spectrum.