Figure 2.

NDCCs Are the Major Catabolites in Arabidopsis.

(A) UV absorption spectrum of At-NCC-1.

(B) UV absorption spectra of At-NDCC-1.

(C) Colorless catabolites of dark-incubated (8 d) leaves of Col-0 were separated by HPLC. A₂₅₄ and A₃₂₀ are shown. For clarity, only the relevant part of the HPLC traces is shown; inset, sums of NDCCs and NCCs accumulating during dark-induced senescence. Values are means of three replicates, and error bars represent sd.

(D) A 600-MHz ¹H-NMR spectrum of At-NDCC-1 (in CD₃OD, 283 K) and constitutional formula of At-NDCC-1. NMR signals indicating hydrogen atoms at C9 and C1 are labeled.