CYP89A9-GFP Likely Localizes to the ER in Arabidopsis Mesophyll Protoplasts.
(A) Transient expression of CYP89A9-GFP and free GFP in protoplasts isolated from green (0 d of dark incubation [ddi]) or senescent (5 ddi) leaves.
(B) Colocalization of CYP89A9-GFP with BiP-RFP in protoplasts isolated from senescent (4 ddi) leaves. GFP, RFP, and chlorophyll autofluorescence was examined by confocal laser scanning microscopy. Note the ER-like membrane network visible in the GFP zoom.
Bars = 20 μm.