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. 2013 May 30;25(5):1868–1880. doi: 10.1105/tpc.113.112151

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Figure 6. — Analysis of Recombinant CYP89A9.

(A) HPLC analysis of assays employing Sf9 microsomes expressing CYP89A9 and ATR1, and epi-pFCC as substrate. HPLC traces at A₃₆₀ after 90 min of incubation at 28°C are shown. For clarity, only the relevant part of the HPLC traces is shown. The inset shows the relative amounts of formed epi-pFDCCs. Values are means of three replicates. Error bars indicate sd.

(B) UV absorption spectrum of epi-pFDCCs produced with recombinant CYP89A9 and chemical constitution of (epi-)pFDCCs. Relevant stereocenters are labeled.

(C) Substrate specificity of CYP89A9. Values are means of three replicates. Error bars indicate sd.

(D) FDCC-to-NDCC isomerization assays. The two most polar epi-pFDCCs produced with recombinant CYP89A9 were incubated at pH 5.0 for up to 120 min and analyzed by HPLC. A₂₅₄ is shown.