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. 2013 May 30;25(5):1868–1880. doi: 10.1105/tpc.113.112151

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Figure 7. — FCC Deformylation Precedes FCC Demethylation.

Colorless catabolites accumulating in dark-incubated (7 d) leaves of Col-0, cyp89a9-1, mes16-1 cyp89a9-1, cyp89a9-1/CYP89A9, mes16-1cyp89a9-1/CYP89A9, and Col-0/PPH_TP-MES16 were separated by HPLC. Relative amounts are means of n replicates, and error bars represent se. Col-0, cyp89a9-1, and cyp89a9-1 mes16-1, n = 3; cyp89a9-1/CYP89A9 and Col-0/PPH_TP-MES16, n = 6; cyp89a9-1mes16-1/CYP89A9, n = 5. For the transgenic lines, replicates correspond to independent transformation events. The significance of differences between Col-0 and Col-0/PPH_TP-MES16 and between cyp89a9-1/CYP89A9 and mes16-1cyp89a9-1/CYP89A9 was determined using a one-tailed t test with Welch correction. n.d., not detected.