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. Author manuscript; available in PMC: 2014 Feb 28.
Published in final edited form as: J Phys Chem C Nanomater Interfaces. 2013 Feb 8;117(8):4268–4277. doi: 10.1021/jp312031u

Table 1.

Comparison of the specificity constant kcat/Km for legumain and cathepsin B, derived from the electrochemical method (i.e. ACV on VACNF NEAs) and the fluorescence assay in solutions.

Enzyme Peptide Sequence kcat/Km (M−1s−1)
Legumain graphic file with name nihms445090t1.jpg (4.3 ± 0.6) × 103
graphic file with name nihms445090t2.jpg (11.3 ± 3.8) × 103
Cathepsin B graphic file with name nihms445090t3.jpg (2.3 ± 1.7) × 104
graphic file with name nihms445090t4.jpg (4.3 ± 0.8) × 104
a

FL: fluorescence; ↓: cleavage site. The fluorescence data were derived from the analysis in Figures S8, S9, S11 and S12.

b

EC: electrochemistry. The electrochemical data were statistical value over at least duplicated experiments.