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. Author manuscript; available in PMC: 2013 Jun 27.
Published in final edited form as: Wiley Interdiscip Rev Dev Biol. 2011 Nov 28;1(1):114–137. doi: 10.1002/wdev.1

Figure 5. MosTIC, MosDEL and MosSCI strategies.

Figure 5

(A) The double-strand break induced by the excision of Mos1 (i) can be repaired by MosTIC using different repair templates to engineer point mutations (ii), fusion proteins (iii), or gene deletions (iv). In the case of MosDEL (v), a unc-119(+) mini-gene is used as a positive selection marker.

(B) The goal of MosSCI is to engineer single copy transgenes at a defined location in the genome. A double-strand break induced by Mos1 excision is repaired by homologous recombination with an in vitro-engineered template which contains the desired transgene in combination with the unc-119(+) mini-gene.