FIG. 4.

ISG56 protein production does not require virus replication and does not correlate with IRF3 hyperphosphorylation. Untransformed HEL fibroblasts (A) or immortalized A549 cells (B) were left untreated (M) or infected with wild-type or UV-inactivated HSV, NDV, or SV. Whole-cell extracts were harvested at 12 or 24 h postinfection, and 40 μg of sample was run on an sodium dodecyl sulfate-9% polyacrylamide gel electrophoresis (SDS-9% PAGE) gel, followed by Western blot analysis. All blots were reprobed with an antibody against β-actin to ensure equal loading (data not shown).