FIG. 6.

Low-multiplicity SV infection induces ISG56 in the absence of detectable IRF3 hyperphosphorylation. A549 cells were infected with replication-competent and UV-inactivated SV at the indicated multiplicities of infection (HAU per 10⁶ cells). Whole-cell extracts were harvested at 8 h postinfection, and 40 μg of sample was run on an SDS-9% PAGE gel, followed by Western blot analysis using antibodies specific for IRF3 and ISG56.