FIG. 7.

IRF3 is differentially modified following low- and high-multiplicity infections in the presence or absence of de novo protein synthesis. A549 cells were either mock infected or infected with SV at a low (2 HAU per 10⁶ cells) or high (40 HAU per 10⁶ cells) multiplicity of infection in the presence or absence of 100 μg of cycloheximide (CHX) per ml. Whole-cell extracts and total RNA were harvested at 8 h postinfection for use in Western blot and RT-PCR analyses, respectively.