TABLE 2.
Effect of LpH-SE and Environ LpH on 263K scrapie infectivity in Syrian golden hamstersa
| Treatment | % Brain inoculated | No. of Sc+ hamsters/no. inoculated | Interval to sacrifice (days) (mean ± SD) |
|---|---|---|---|
| LpH-SE | 0.1 | 4/4 | 89 ± 0 |
| 0.01 | 4/4 | 100 ± 4.5 | |
| 0.001 | 4/4 | 133 ± 38 | |
| None | 0.1 | 4/4 | 82 ± 0 |
| 0.01 | 4/4 | 89 ± 0 | |
| 0.001 | 4/4 | 92 ± 3 | |
| LpH-SE + autoclave | 0.1 | 0/4 | *b |
| Environ LpH | 0.1 | 0/3 | *b |
| Environ LpH + autoclave | 0.1 | 0/4 | *b |
a
Data relative to Environ LpH is taken from an earlier study (4) and is shown for comparison to LpH-SE. The Environ LpH result for 1% LpH and 0.1% brain homogenate is shown. Hamsters were inoculated intracerebrally with 50 μl of the indicated suspension of LpH-treated or untreated brain. The interval to sacrifice is the time from inoculation to definite clinical scrapie. Sc+, scrapie positive.
b
*, no clinical scrapie disease during a 15-month postinoculation observation period.