Table 2. Primer sequences, the lengths of the amplified gene segments, and the conditions for the real-time PCR reactions.

Gene	Forward primer sequence Reverse primer sequence	Length of amplified gene segment	Conditions for real-time PCR reactions
GAPDH	5′-TATGATTCTACCCACGGCAA-3′	154 base pairs	[Mg2+] = 3.5mM
	5′-CAGTGGACTCCACAACATAC-3′		Annealing temperature = 60°C
Collagen Type I	5′-TGGTTCTCCTGGCAAAGAT-3′	232 base pairs	[Mg2+] = 3.5mM
	5′-ATCACCGGGTTCACCTTTA-3′		Annealing temperature = 60°C
Collagen Type III	5′-ACAGCAGCAAGCTATTGAT-3′	156 base pairs	[Mg2+] = 3.5mM
	5′-GGACAGTCTAATTCTTGTTCGT-3′		Annealing temperature = 60°C
MMP2	5′-AGCTACTTCTTCAAGGGTG-3′	150 base pairs	[Mg2+] = 4.0mM
	5′-GTGTGCAGAAGGCAATG-3′		Annealing temperature = 60°C
MMP3	5′-GGAGAGGCTGACATAAAGATT-3′	161 base pairs	[Mg2+] = 4.0mM
	5′-GATGTATCGCTTGTCCATTG-3′		Annealing temperature = 60°C
MMP9	5′-TGCCTGAGACTGGAGAG-3′	159 base pairs	[Mg2+] = 4.0mM
	5′-GCAAGTCTTCCGAGTAGTT-3′		Annealing temperature = 60°C
MMP13	5′-TACAACTTGTTCCTTGTCGC-3′	166 base pairs	[Mg2+] = 4.0mM
	5′-CTGGGCCATAGAGAGACT-3′		Annealing temperature = 60°C
MMP14	5′-AAGCACTGGGTGTTTGAT-3′	169 base pairs	[Mg2+] = 4.0mM
	5′-GTTCCTCGTTGAAACGGT-3′		Annealing temperature = 60°C
TIMP1	5′-CAGCGAGGAGTTTCTGG-3′	157 base pairs	[Mg2+] = 4.0mM
	5′-GGTAAACACTGTGCACCC-3′		Annealing temperature = 60° C
TIMP2	5′-GAGGAAAGAAGG AGTATCTCATTG-3′	195 base pairs	[Mg2+]=4.0mM
	5′-CCGGAGACGAGATATAGC-3′		Annealing temperature = 60° C