FIGURE 2:

Sis1 is essential for the degradation of several misfolded Doa10 substrates. (A) Degradation of Ndc10-2 in sis1-85 cells. Cells incubated at 24°C were shifted to 37°C at 30 min before the CHX-chase experiment and kept at 37°C thereafter. (B) Vma12-DegAB is rapidly degraded in ydj1-151 cells but stabilized in sis1-85 cells. (C) Sis1 activity is required for the elimination of GFP-DegAB but not of mutant carboxypeptidase Y (CPY*). (D) Degradation of Deg1-Vma12-protein A (Deg1-VP) requires intact Sis1. (E) Degradation of the Doa10 native substrate, Ubc6, does not require Sis1 activity.