FIGURE 5:

cAMP and folate use different receptors and G protein couplings but the same GEF/Ras pathway to mediate AKT and PKBR1 phosphorylation. Strains deficient for different signaling components were stimulated with 50 μM folate in starvation buffer or with 10 μM cAMP after differentiation for 6 h in shaking culture with cAMP pulses. TORC2 phosphorylation of AKT and PKBR1 was assayed by immunoblot at the times indicated. Genotypes in bold indicate cells that are unresponsive to active TORC2 by either cAMP or folate.