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. 2013 Jul 1;24(13):2146–2155. doi: 10.1091/mbc.E13-03-0130

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© 2013 Liao et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 6: — RasC activation does not cross-adapt to different chemoattractants. (A) Cells stimulated with saturating doses of cAMP remain responsive to folate. FLAG-RasC–expressing cells were stimulated with 10 μM cAMP and then stimulated with either 10 μM cAMP or 50 μM folate at 75 s. RasC-GTP levels were determined by interaction-specific affinity and normalized to total RasC by α-FLAG immunoblot assay at the times indicated. For quantification, see Supplemental Figure S5A. (B) Cells stimulated with saturating doses of folate remain responsive to cAMP. FLAG-RasC–expressing cells were stimulated with 50 μM folate and then stimulated with either 50 μM folate or 10 μM cAMP at 75 s. RasC-GTP levels were determined by interaction-specific affinity and normalized to total RasC by α-FLAG immunoblot assay at the times indicated. For quantification, see Supplemental Figure S5B.