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. 2013 Jun 27;9(6):e1003599. doi: 10.1371/journal.pgen.1003599

Figure 5. Residual NHEJ in the chromosomal suicide deletion assay with catalytically defective Dnl4.

Figure 5

(A) Diagram of the suicide deletion chromosomal assay used to determine NHEJ efficiency in panels (B) to (D). (B) Cells were pre-grown to stationary phase in synthetic defined medium prior to plating to galactose. When indicated, the chromosomal dnl4 allele was complemented with a plasmid bearing wild-type DNL4 (pDNL4). (C) Cells were pre-grown to log phase in YPA-Glycerol. (D) Epistasis analysis of Dnl4 catalytic mutants with nej1Δ. Results are the mean ± standard deviation of at least three independent experiments.