Figure 6. End joining profiles after extensive HO recleavage depend on Dnl4 status.

(A) Fractions of different ILV1-cs joint categories after 24 hours of HO induction, showing preserved imprecise joining with K466A. “Precise sequence” is the same as the input allele prior to DSB formation, “major imprecise joints” had a frequency ≥0.1% in any tested strain, and “other joints” are the remainder. (B) Individual major imprecise joints that showed significantly different frequencies between wild-type and K466A. The left half shows joints that had increased frequency in K466A as compared to wild-type. The right half shows joints that had a decreased frequency. Red bars, joint fraction in K466A; green bars, joint fraction in wild-type. See Figure S5 for a description of the joint identifiers and File S1 for their sequences.