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. 2013 Jun 27;9(6):e1003599. doi: 10.1371/journal.pgen.1003599

Figure 7. Cdc9 recruitment to a chromosomal DSB during the NHEJ repair phase.

Figure 7

Yeast strains bearing 13Myc-tagged Cdc9 with the indicated Dnl4 mutations and the GAL1-cs allele were pre-grown to log phase in YPA-Glycerol (A, B) or post-diauxic phase (C, D) and treated with 2% galactose to induce HO expression and DSB formation. (A) and (C) show the fraction of intact GAL1-cs HO cut sites and thus the extent of DSB formation and repair over time as determined by flanking PCR. (B) and (D) show the corresponding enrichment of 13Myc-Cdc9 at the GAL1-cs DSB as determined by ChIP from the same samples as (A) and (C), respectively. ‘No ab’ indicates background signal obtained without antibody addition during immunoprecipitation. Results are the mean ± standard deviation of two independent experiments.