Figure 3. Sanger-based verification of two novel methylated positions in S. solfataricus rRNA identified using RNA-seq.
Bisulfite-converted sequences were amplified using amplicon-specific primers and sequenced. (A) Position C1369 in the S. solfataricus 16S rRNA. (B) Position C2643 in the S. solfataricus 23S rRNA.