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. 2013 Jun 27;9(6):e1003446. doi: 10.1371/journal.ppat.1003446

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© 2013 Strijbis et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A): Colocalization of BTK-mCherry and PH-BTK-GFP that binds to PI(3,4,5)P₃ at 30 and 90 minutes of coincubation with Candida-BFP showing phagocytosis of yeast and hyphae, respectively. (B): Colocalization of mCherry-Vav1 and PH-BTK-GFP at 30 and 90 minutes of coincubation with Candida-BFP. (C): Localization of mCherry-Syk and PH-BTK-GFP at 30 and 90 minutes of coincubation with Candida-BFP. (D): Localization of BTK-mCherry and C1-PKCδ-GFP at 30 and 90 minutes of coincubation with Candida-BFP. (E): Localization of mCherry-Vav1 and C1-PKCδ-GFP at 30 and 90 minutes of coincubation with Candida-BFP. (F): Localization of mCherry-Syk and C1-PKCδ-GFP at 30 and 90 minutes of coincubation with Candida-BFP. White arrows indicate areas of co-localization, while red and green arrows indicate areas of speciation. Experiments were performed at least three times, representative micrographs are shown.