Figure 7. SAMHD1 augments HSV-1 infection in human primary DCs and MDMs.

Primary mature DCs were inoculated with medium containing vehicle, Vpx− VLP, or Vpx+ VLP for 48 h and then infected with HSV-1 KOS at a MOI of 0.1. Cell lysates were generated at 48 hpi and (A) western blot analysis for SAMHD1 and tubulin were performed. (B) Real-time PCR for HSV-1 DNA levels were determined 48 hpi. (C) Primary human MDMs were pretreated with VLPs before infecting with HSV-1 F strain (0.5 PFU/cell). Infection was monitored for intracellular staining of viral gB protein by FACS analysis and the data graphed as a percentage of gB positive MDMs.