Figure 6. Acute treatment with Fasudil (panels a and d) or PD98059 (b and c) attenuates diabetes-induced elevation in phosphorylation of ROCK and ERK1/2 in the mesenteric vascular bed.

Panels a) and c) are representative Western blots showing phosphorylated (p-) levels of the stated proteins in the isolated mesenteric bed from normal controls (C), diabetic (D) and diabetic mesenteric bed treated with fasudil (10⁻⁶M;+FAS) or PD 98509 (10⁻⁶M;+PD) respectively. Panels b) and d) are densitometry histograms showing phosphorylated (p-) levels of the stated proteins normalized to actin. N = 5; Mean±SD. Asterisk (*) indicates significantly different (p<0.05) mean values from normal non-diabetic rats (C) whereas hash (#) indicates significantly different mean values (p<0.05) from diabetic rats (D).