Skip to main content
. 2013 Jun 27;8(6):e67813. doi: 10.1371/journal.pone.0067813

Figure 10. ROCK is an downstream stream effector of ERK1/2 signaling in diabetes-induced vascular dysfunction.

Figure 10

The effect of treatment with fasudil (FAS) or PD98509 (PD) acutely in the isolated mesenteric vascular bed (A) or B) in cultured VSMC on phosphorylation (p-) of ROCKII and ERK1/2. Shown in panel i) of each figure is a respresentative Western blot and in panels ii-iii) are the densitometry histograms showing phosphorylated (p-) levels of the stated proteins normalized to actin. C = non-diabetic controls, D = Diabetic rats; NG and HG refers to VSMC grown in normal (5.5mM) D-glucose or high glucose (25.5mM D-glucose) respectively. N = 5; Mean±SD. Asterisk (*) indicates significantly different (p<0.05) mean values from normal non-diabetic rats (C) whereas hash (#) indicates significantly different mean values (p<0.05) from diabetic rats (D).