Figure 1. Adoptive immune therapy in mice requires both T-bet and Eomes.
B6-LY5.2/Cr mice were challenged with 3×105 B16F0 cells i.d. 6 days later, mice were irradiated at 500 rad. On day 7, the mice were adoptively transferred with 5×105 WT, T-bet−/−, Eomes−/−, or T-bet/Eomes DKO pmel-1 T cells which have been cultured in Th1 condition for 3 days. Tumor growth was monitored every two days. 5 mice were in each group. The no-transfer group did not receive any T cells. *P<0.05, determined by Mann-Whitney Test comparing recipients infused with DKO and WT T cells.