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. 2013 Jun 27;8(6):e68255. doi: 10.1371/journal.pone.0068255

Figure 1. Rab37 GTPase is expressed in β-cells and is localized on insulin-containing granules.

Figure 1

A. Rab37 expression in insulinoma cell lines (in βTC3, MIN6B1 and INS-1E cells), rat cortex and human pancreatic islets. Total lysates were immunoblotted with an anti-mouse Rab37 polyclonal antibody (detected band at 25 kDa). Protein loading was verified with an anti-mouse actin monoclonal antibody. B. Insulinoma cell lines MIN6B1 (upper panel) and INS-1E (lower panel) were stained for Rab37 (a,d) and either insulin (b) or the GTPase Rab3a (d). After immunocytochemistry, the cells were observed on a confocal microscope for colocalization (c,f). Insets represent higher magnifications of the boxed regions. C. MIN6B1 (upper panel) and INS-1E (lower panel) cells were co-transfected with Rab37wt-EGFP (a,d) and with NPY-mRFP (b,e). Two days later, the cells were fixed and observed on a confocal microscope. Rab37wt-EGFP co-localizes with NPY-containing large dense core granules (c,f). Insets represent higher magnifications of the boxed regions.