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. 2013 Jun 27;8(6):e68255. doi: 10.1371/journal.pone.0068255

Figure 2. Rab37 knock-down impairs hormone secretion induced by secretagogues.

Figure 2

A. The level of endogenous Rab37 (upper panel) and exogenously expressed Rab37-EGFP (lower panel) were estimated by immunoblotting in cells transfected with control shRNA, shRab37 (1) or (2). Equal loading of the lanes was verified using an antibody against actin. B. To assess hormone release, the cells were transiently co-transfected with a plasmid encoding the human growth hormone and with plasmids driving the expression of shRNAs against GFP (control) or with two different shRNAs against Rab37 (shRab37 (1) or (2)). Three days later, the cells were tested for hormone release. For this, they were maintained at 2 mM glucose, (open bars) and then incubated in stimulating solutions (filled bars) containing 20 mM glucose and the cAMP-raising agents Forskolin and IBMX. The amount of hGH expressed by the cells and released in the medium was quantified by ELISA. Hormone secretion under basal and stimulatory conditions is given as % of hGH cellular content. The results are the means of five independent experiments ± SEM. ** p<0.01, *** p<0.001. One-way ANOVA.