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. 2013 Jun 27;8(6):e68255. doi: 10.1371/journal.pone.0068255

Figure 3. Rab37 participates in docking of insulin-containing granules.

Figure 3

A. Representative TIRF images showing docked NPY-mRFP containing granules in MIN6B1 cells transfected either with control shGFP, shRBa3a, shRab27a or with shRab37 (2). B. Analysis of the docked granules detected by TIRF illumination in the experiments shown in A. Histograms show the quantification of the number of docked granules per surface (µm2). Results are means ± SEM (shGFP, n = 41 cells; shRab37 (2), n = 38 cells; shRab3a, n = 33 cells; shRab27, n = 37 cells). * p<0.05 one-way ANOVA test. C. Quantification of the exocytotic events from NPY-mRFP transfected MIN6B1 cells expressing control shGFP, shRab3a or shRab37 (2). Exocytosis was triggered when indicated (Stimulation) by treating the cells with glucose, Forskolin and IBMX. Histograms represent the number of fusion events counted every 60 s (n = 12). The results shown are the means ± SEM. p * <0.05 unpaired t-test.