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. 2013 Jun 27;8(6):e68255. doi: 10.1371/journal.pone.0068255

Figure 5. Effect of prolonged exposure to physiopathological conditions on Rab37 expression.

Figure 5

Rab37 and Rab3a mRNA levels in MIN6B1 cells exposed for 24h to inflammatory cytokines (IL-1β alone or a mix of IL-1β TNFα, and IFNγ) (A), to palmitate (0.5 mM, Palm) (B) or for 72 h to 2 mM native or mildly oxidized LDL (oxLDL) (C) were quantified by qRT-PCR. MIN6B1 cells were transfected with control siRNA (siGFP, open bars) or siICER (filled bars) and cultured with either BSA or 0.5 mM palmitate for 48 h (D) or with vehicle, 2 mM native or mildly oxidized LDL for 72 h (E). The mRNA levels of Rab37 and granuphilin/Slp4 were quantified by qRT-PCR. The mRNA levels were normalized either against 18S (A–C) or against the housekeeping gene acidic ribosomal phosphoprotein P0 (Rplp0) (D–E). The expression levels in control cells were set to 100%. Data are means of ± SEM of at least 3 independent experiments (* p<0.05; ** p<0.01, one-way ANOVA).