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. 2013 Jun 27;8(6):e68055. doi: 10.1371/journal.pone.0068055

Figure 5. Hc-TeTx-dependent Akt signaling depends on nSMase.

Figure 5

A) Phosphorylation of Akt in serine 473 in CGN were tested after 10 nM Hc-TeTx treatment for 15 min in low potassium conditions (5 mM), with or without GW4869 (20 µM) pretreatment for 1 h, using 50 ng/mL BDNF for 15 min as positive control and an phosphospecific antibody in western blot. Loading control was assessed using an antibody against total Akt. Experiment was performed in triplicate. B) Films were scanned and signal quantified using Image J software. The values are represented in the bar graph in percentage respect to the control condition. Error bars represent SD.