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. 2013 Jun 27;8(6):e67294. doi: 10.1371/journal.pone.0067294

Figure 2. miR-30a and DLL4 played opposite roles in the proliferation and migration of HUVEC-C cells.

Figure 2

(A) DLL4-transfected HUVEC-C cells gained more proliferation capacity than control cells detected by MTS assay (left panel), while miR-30a suppressed HUVEC-C cell proliferation relative to control cells (middle panel). HUVEC-C cells were co-transfecting with either miR-30a and DLL4 or DLL4 and pcDNA3.0 and showed that miR-30a partially inhibited the DLL4-stimulated proliferation (right panel). (B) HUVEC-C cells transfected with DLL4 vector and miRNAs control vector (pcDNA) gained more migration capacity than cells transfected with double negative control vectors (XL6+pcDNA). However, HUVEC-C cells transfected with DLL4 vector and miR-30a gained less migration capacity than cells transfected with DLL4 and pcDNA vectors. (C) The numbers of migrated cells in the four groups are shown in bars. Data are presented as the mean ± SEM. *statistics significant.