TABLE 3.
Propagation of GSS(P101L) prions in transgenic mice
| Inoculuma | Treatment | Time to disease (days ± SEM) | n/n0b | Time elapsed (days) |
|---|---|---|---|---|
| Tg196c | None | 478 ± 37 | 6/7 | >500 |
| PTA | 432 | 1/8 | >550 | |
| PK/PTA | 468 ± 22 | 2/9 | >500 | |
| PTA/PK | 483 | 1/9 | >500 | |
| Tg2866d | None | 305 ± 17 | 10/10 | |
| PTA | 278 ± 16 | 10/10 | ||
| PK/PTA | 262 ± 12 | 8/8 | ||
| PTA/PK | 226 ± 8 | 10/10 | ||
| Tg196(2866)e | None | 351 ± 13 | 8/8 | |
| PTA | 294 ± 4 | 9/9 | ||
| PK/PTA | 286 ± 3 | 10/10 | ||
| PTA/PK | 279 ± 7 | 9/9 | ||
| Tg196(196(301V))f | None | 144 ± 4 | 6/7 | >550 |
| PTA | 148 ± 6 | 7/7 | ||
| PK/PTA | 126 ± 3 | 5/5 | ||
| PTA/PK | 131 ± 3 | 8/8 |
All transmissions were carried out using Tg(MoPrP,P101L)196/Prnp0/0 mice as recipients. For each inoculum series, a pool of at least three brain homogenates was split among the following treatments: none, untreated; PTA, sodium phosphotungstate precipitation; PK/PTA, PK digestion followed by PTA precipitation; PTA/PK, PTA precipitation followed by PK digestion.
Number of ill animals (n) over number of animals under observation (n0).
Samples from clinically normal Tg(MoPrP,P101L)196/Prnp0/0 mice age-matched with ill Tg196(2866) mice at ∼300 days of age.
Tg2866 corresponds to spontaneously ill Tg(MoPrP,P101L)2866/Prnp0/0 mice at ∼130 days of age.
Tg196(2866) corresponds to ill Tg(MoPrP,P101L)196/Prnp0/0 mice (∼300 days of age) following inoculation with brain homogenates from spontaneously ill Tg2866 mice.
Tg196(196(301V)) inoculum was obtained from the secondary transmission of 301V prions into Tg(MoPrP,P101L)196/Prnp0/0 mice (see Table 1).