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. 2013 Jun 27;8(6):e68125. doi: 10.1371/journal.pone.0068125

Figure 3. NADH reduces the Po of the mitochondrial large-conductance Ca2+-regulated potassium channel at positive voltages.

Figure 3

A. Single-channel recordings of the mitoBKCa channel activity in symmetric 150/150 mM KCl isotonic solution (200 µM Ca2+) at +40 and −40 mV under control conditions, after the addition of 200 µM reduced nicotinamide adenine dinucleotide (NADH) and after perfusion. B. Single-channel recordings of the mitoBKCa channel activity in a symmetric 150/150 mM KCl isotonic solution (200 µM Ca2+) at +40 mV under control conditions, after the addition of 250 nM rotenone (Rot) and 200 µM NADH plus 250 nM Rot, and after perfusion. C. Single-channel recordings of the mitoBKCa channel activity in symmetric 150/150 mM KCl isotonic solution (200 µM Ca2+) at +40 mV under control conditions, after the addition of 1 µM antimycin A (Anti) and 200 µM NADH plus 1 µM Anti and after perfusion. D. Analysis of Po under the conditions described in A, B and C. *P<0.003 vs. the control.