Figure 2. Direct downstream gene targets of the C/EBP-ε transcription factor.

Several C/EBP-ε target genes were significantly downregulated in both Evi1 overexpressed leukemic cell lines. The x-axis is the significantly downregulated gene and the y-axis denotes reads per million on RNA-Seq analysis. a) In DA-1 Evi1 overexpressed cells, Serpinb2 had an 11.4-fold decrease (p = 0.0006), Myc had a 2.5-fold decrease (p = 0.04), Lcn2 had a 3.4-fold decrease (p = 0.001), Epx had a 13.5-fold decrease (p = 0.0003). b) In DA-1 Evi1 overexpressed cells, although the overall level of transcription for Mmp8 and Prg2 were reduced compared to the other significantly downregulated C/EBP-ε gene targets, they exhibited a very large significant fold change in the EVI leukemic cells. Mmp8 had a 13.5-fold decrease (p = 0.02), and Prg2 had a19.0-fold decrease (p = 0.003) in the EVI1 leukemic cells compared to the knockdown. c) In NFS-60 Evi1 overexpressed cells, Lcn2 had a 3.9-fold decrease (p = 0.001), Mmp8 had a 12.7-fold decrease (p = 6.5E⁻⁵), and Serpinb2 had an 11.5-fold decrease (p = 5.8E⁻⁵).