Figure 6. Up-regulated miR-182 and down-regulated miR-10a expression in Foxp3⁺ cells is required for Th2-Tregs to suppress Th2 response and Th1-Tregs to suppress Th1 response in vivo, respectively.

In vitro-polarised Th1 or Th2 T effector (Teff) cells were adoptively transferred with Th1-Treg (CD4⁺Foxp3^gfp+) or Th2-Treg isolated from the lungs of mice with Th1 or Th2-driven pulmonary inflammation, respectively and as indicated. Co-transferred Foxp3+ Treg cells were either mock transfected or transfected with miR-10a mimics or miR-182 inhibitors, as indicated. (A) Recruitment of donor Treg cells to the lungs of recipient mice, as a percentage of total CD4⁺ cells in the lung. (B) Broncho-alveolar infiltrates of mice one day following final OVA challenge. (C) Local lymph nodes were isolated and re-stimulated with OVA. IFNγ or IL-5 was measured in the supernatants after 3 days. (D) Lungs were removed, sectioned and stained with H&E for pathology. One of 2 individual experiments shown. p-value = 0.05 with data expressed as mean ±SEM.