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. 2004 Feb;78(4):1697–1705. doi: 10.1128/JVI.78.4.1697-1705.2004

FIG. 1.

FIG. 1.

LMP2A induces the phosphorylation of Akt in Ramos and HSC-39 cells. (A) Ramos and HSC-39 cells were stably retrovirally transduced with LMP2A and vector control expression constructs. After 24 h of serum starvation, whole-cell extracts were separated by SDS-polyacrylamide gel electrophoresis. The levels of expression of LMP2A (54 kDa) and phosphorylated Akt (serine 473) (P-Akt) (60 kDa) in parental (P), vector control (V), and LMP2A-expressing Ramos and HSC-39 cells were determined by immunoblotting. LCL, lymphoblastoid cell line. LCL was used as the positive control for LMP2A and Akt phosphorylation. (B) Effect of serum starvation on the phosphorylation of Akt in LMP2A-expressing Ramos and HSC-39 cells. Parental (P), vector control (V), and LMP2A-expressing Ramos and HSC-39 cells were switched to RPMI 1640 medium without FBS (−), harvested at the indicated times, and analyzed by immunoblotting for P-Akt. The lower panels show equal loading of proteins and the expression of total Akt (T-Akt) (60 kDa).